Genotypic Characterization of Sulfur-Oxidative Desulfurizing Bacterial Strains Isolated from Mexican Refineries
BUSTOS-JAIMES I. (Instituto Mexicano del Petroleo(1)) AMADOR G. (Instituto Mexicano del Petroleo(1)) CASTORENA G. (Instituto Mexicano del Petroleo(1)) LE BORGNE S. (Instituto Mexicano del Petroleo(1))
We performed a genotypic characterization of new sulfur selective dibenzothiophene (DBT) desulfurizing strains which have been isolated from hydrocarbon contaminated soils originating from Mexican refineries. We compared them to two reference strains (strains Rhodococcus erythropolis IGTS8 and R. erythropolis X309). These reference strains have been extensively studied and are protected by patents as well as all biodesulfurization (BDS) processes derived from them. The aim of the present study was to use molecular tools to characterize these new isolates and determine whether they were different from the other patented strains. The characterization methods included 16S rDNA gene partial sequencing, PCR amplification of fragments associated to the desulfurization phenotype, restriction analysis and randomly amplified polymorphic DNA (RAPD). Partial 16S rDNA gene sequence analysis showed that the isolated strains were different from R. erythropolis IGTS8 although they contained the same desulfurizing dszABC genes. The RAPD patterns were also different from those of the two reference strains used in this study confirming that the isolated bacteria were different from the already patented desulfurizing strains R. erythropolis IGTS8 and X309.
(1) Eje Central Lazaro Cardenas 152 07730 Mexico D.F. Mexico